In vivo determination of carotenoid resonance excitation profiles of Chlorella vulgaris, Haematococcus pluvialis, and Porphyridium purpureum

authored by: Matthias Koch, Ann-Kathrin Kniggendorf, Merve Meinhardt-Wollweber, Bernhard Roth
Abstract: Under stress, certain species of algae produce pigments such as astaxanthin, β-carotene, or phycoerythrin for protection against photosynthethic stress. These pigments are of high commercial demand, for example, as food additives. Measuring and maintaining stress conditions precisely are necessary for efficient pigment synthesis in algae cultures. We present resonance excitation maps of three common species of algae, Chlorella vulgaris, Haematococcus pluvialis, and Porphyridium purpureum, exploring the feasibility of resonance Raman spectroscopy for stress monitoring in vivo. The resonance excitation profiles for individual Raman lines of carotenoids extracted from the resonance excitation maps of different stress states are a helpful guide to pick optimal excitation wavelengths for maximum resonance enhancement in living algae cultures of these species under laboratory conditions.
Organisation(s): Hannover Centre for Optical Technologies (HOT)
Type: Article
Journal: Journal of Raman Spectroscopy
Volume: 49
Pages: 404-411
No. of pages: 8
ISSN: 0377-0486
Publication date: 06.12.2017
Publication status: Published
Peer reviewed: Yes
ASJC Scopus subject areas: General Materials Science, Spectroscopy
Electronic version(s): https://doi.org/10.15488/3358 (Access: Open)
https://doi.org/10.1002/jrs.5292 (Access: Closed)

BibTeX

@article{708df7adea504c79baa0b1a5eef4b935,
title = "In vivo determination of carotenoid resonance excitation profiles of Chlorella vulgaris, Haematococcus pluvialis, and Porphyridium purpureum",
abstract = "Under stress, certain species of algae produce pigments such as astaxanthin, β-carotene, or phycoerythrin for protection against photosynthethic stress. These pigments are of high commercial demand, for example, as food additives. Measuring and maintaining stress conditions precisely are necessary for efficient pigment synthesis in algae cultures. We present resonance excitation maps of three common species of algae, Chlorella vulgaris, Haematococcus pluvialis, and Porphyridium purpureum, exploring the feasibility of resonance Raman spectroscopy for stress monitoring in vivo. The resonance excitation profiles for individual Raman lines of carotenoids extracted from the resonance excitation maps of different stress states are a helpful guide to pick optimal excitation wavelengths for maximum resonance enhancement in living algae cultures of these species under laboratory conditions.",
keywords = "Chlorella vulgaris, excitation profile, Haematococcus pluvialis, Porphyridium purpureum, resonance Raman spectroscopy",
author = "Matthias Koch and Ann-Kathrin Kniggendorf and Merve Meinhardt-Wollweber and Bernhard Roth",
note = "Publisher Copyright: Copyright {\textcopyright} 2017 John Wiley & Sons, Ltd. Copyright: Copyright 2018 Elsevier B.V., All rights reserved.",
year = "2017",
month = dec,
day = "6",
doi = "10.15488/3358",
language = "English",
volume = "49",
pages = "404--411",
journal = "Journal of Raman Spectroscopy",
issn = "0377-0486",
publisher = "John Wiley and Sons Ltd",
number = "3",
}